Stable Cell Line Development Services
Development of stable cell lines capable of producing recombinant proteins and monoclonal antibodies at high and reliable levels can be critical to a biopharmaceutical research project. GenScript is proud of offering its years of demonstrative and professional custom services in creation and selection of stable clones for protein production. GenScript's expert staff and state-of-the-art facilities are fully equipped to execute complete studies on genetic and productive stability in the presence and absence of selective agents.
Stability Tested For Every Cell Line Generated By GenScript!
- Research Cell Line
- Production Cell Line
- Competitive Advantages
- Related Services
Research Cell Line (SC1260)
| Services |
Description |
Deliverables |
Timeline |
| 1. Gene synthesis & Subcloning (Optional) |
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- Sequence report
- Construct(s) report
|
4 weeks |
| 2.Transient expression evaluation |
- Transient expression analysis
- Detect the expression level by ELISA,WB or IEF
|
|
1–2 weeks |
| 3.Cell line development using mammalian cells |
- Stable transfection and screening
- 3 passages evaluation for top 5 clones
- Deliver 2 vials each of top 1 clones
|
- Top 1 stable clone
- Report
- QC data
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6–7 weeks |
Stable Research Cell Line (SC1505)
| Services |
Description |
Deliverables |
Timeline |
| 1. Gene synthesis & Subcloning (Optional) |
|
- Sequence report
- Construct(s) report
|
4 weeks |
| 2.Transient expression evaluation |
- Transient expression analysis
- Detect the expression level by ELISA,WB or IEF
|
|
1–2 weeks |
| 3.Stable cell line development using mammalian cells |
- Stable transfection and screening
- 10 passages evaluation for top 10 clones
- Deliver 3 vials each of top 3 clones
|
- Top 3 stable clones
- Report
- QC data
|
9–11 weeks |
|
Stable Production Cell Line (SC1503)
| Services |
Description |
Deliverables |
Timeline |
| 1. Gene synthesis & Subcloning (Optional) |
|
- Sequence report
- Construct(s) report
|
4 weeks |
2.Transient expression
evaluation |
- Transient expression analysis
- Detect the expression level by ELISA,WB or IEF
|
|
2–3 weeks |
| 3.Stable cell line development using mammalian cells |
- Stable transfection and screening
- MTX amplification and screening
- 10 passages evaluation for top 10 clones
- Deliver 3 vials each of top 3 clones
|
- Top 3 stable clones
- Report
- QC data
|
21–26 weeks |
Case Study
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Fig. MTX amplification for CHO cells expressing a Fc fusion protein. The expression level of the Fc fusion protein increases more than 40-fold after 3 rounds of genomic amplification using MTX. |
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 A strong commitment of scientific teams with many years of experience in developing highly productive stable cell lines
Multiple parental cells: 293, CHO, BHK, or NS0 cells, or your specified cell lines
Strict expression stability evaluation and wide range of functional testing assays
State-of-the-art facilities capable of supporting large-scale protein production
Over hundreds of cell lines have been delivered on-time
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1. Mammalian Expression: GenScript's proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.
2. Process Development and Scale-up: GenScript can optimize the system's growth and production media, also providing a thorough analysis of the growth rate, subcultivation ratio, cell mass, and specific and volumetric productivity using productivity-enhancing conditions. Also, a scale-down model can be applied to predict cell performance. GenScript employs a novel and highly productive proprietary system which is featured with predictive high-throughput.
3. Bacterial Expression: GenScript has developed BacPowerTM technologies to tackle hard-to-express and hard-to-dissolve proteins. Guaranteed package (SC1253): 3 mg purified protein.
4. Yeast Expression: Our yeast expression system employs stable and durable production strains that resemble mammalian protein processing, incorporating features of high yield, high productivity, and dramatic reductions in costs and time requirements.
5. Insect Cell/Baculovirus Expression: GenScript has pioneered methods for the secretion of recombinant proteins from baculovirus-infected insect cells. Our services are suitable for academic institutions and both the pharmaceutical and biotechnology industries.
6. Purification: Comprehensive purification method and tag to meet you specific needs. The purification tag can be removed via a specific protease, and the protein is further purified by gel-filtration.
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Quotations and Ordering:
 To request a quotation, please download and complete the Quotation Form, and send it back by email or fax.
- For our R&D mammalian stable cell line development service, please download
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- For our protein production mammalian stable cell line development service, please download
 .
To order, please send the completed Quotation Form to us by email or
fax with a formal PO (Purchase Order) or credit card information. You
can also submit PO/credit card information by phone or via our secure online messaging system.
If you are also submitting samples, please mail them together with a hard copy of the completed Quotation Form to the following address: BioProcess Services, GenScript, 860 Centennial Avenue., Piscataway, NJ 08854, US.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
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