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precast gel

Express™ PAGE Gels

GenScript Express™ PAGE Gels is a high performance precast polyacrylamide gel system with a variety of acrylamide percentages. With GenScript proprietary technology, Express™ PAGE Gels are designed as an easy-to-use tool. The unique gel formulation eliminates the "smile" and poor resolution of other gel types. You'll produce reliable separation results every time. In addition, proteins transfer from Express™ PAGE Gels is more efficient, leading to more sample on your membrane for higher detection signals during Western analysis.

Key Features:

Easy to use: simple to set up, without cumbersome combs or tapes.
High Resolution: unique formulation allowing for superior protein band resolution.
Fast Running time: 45 minutes or less.
High Transfer Efficiency: 30 min at semi-dry condition.
Long Shelf Life: up to 12 months at 4°C.

Product Details
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	Cat. No.	Separation Range	% Acrylamide	Wells	Vol.	Size	Price	Quantity
	MG420W10*	205 - 6.5 kDa	4-20%	10	50 μl	1 Box of 10	$68.00
	MG420W12			12	30 μl	1 Box of 10	$68.00
	MG420W15			15	25 μl	1 Box of 10	$68.00
	MG816W10	205 - 14 kDa	8-16%	10	50 μl	1 Box of 10	$68.00
	MG816W12			12	30 μl	1 Box of 10	$68.00
	MG816W15			15	25 μl	1 Box of 10	$68.00
	MG012W10		12%	10	50 μl	1 Box of 10	$68.00
	MG012W12			12	30 μl	1 Box of 10	$68.00
	MG012W15			15	25 μl	1 Box of 10	$68.00
	MG010W10	205 - 24 kDa	10%	10	50 μl	1 Box of 10	$68.00
	MG010W12			12	30 μl	1 Box of 10	$68.00
	MG010W15			15	25 μl	1 Box of 10	$68.00
	MG008W10	205 - 45 kDa	8%	10	50 μl	1 Box of 10	$68.00
	MG008W12			12	30 μl	1 Box of 10	$68.00
	MG008W15			15	25 μl	1 Box of 10	$68.00

	Cat. No.	Name	Size	Price	Quatity
	MB01015	5X Sample Buffer	5 ml	$24.50
	M00138^*	Express Gel Running Buffer Powder	1 Box (5/PK)	$38.00
	M00139^*	Transfer Bufffer Powder	1 Box (10/PK)	$58.00
^*To make 1000 ml 1X Running Buffer or Transfer Bufffer per package

Note: GenScript Express™ PAGE gels are formulated using 1X Tris-MOPS-SDS buffer (PH 6.8). For best results, please use GenScript running buffer (Cat. No. M00138). This buffer will ensure excellent separation and high-resolution bands. Otherwise, please prepare buffers according to our protocol.
* Free samples available.

Protein Migration Table:

The Protein Migration Table as following can also help you to choose the appropriate gels for your protein electrophoresis. The data of this table is from gels run at 140 volts for 45 min. At 140 volts, the Bromophenol Blue band will reach the bottom of the gels in about 30 min.

precast gel

Recipe of 5X Sample Buffer*:

SDS 1.0 g

Glycerol 5.0 ml

Bromophenol Blue 25 mg

Tris Base 150 mg (adjust the PH to 6.8)

2-Mercappethanol 1.0 ml

Deionized Water To 10 ml

Recipe of 10X Running Buffer* and 20X Transfer Buffer*:

10X Running Buffer (Poweder) 20X Transfer Buffer (Poweder)

Tris Base 60.6 g 60.0 g

Bicine 81.6 g

MOPS 104.6 g

SDS 10.0 g

EDTA 3.0 g

Deionized Water To 1000 ml 1000 ml

Note To make 1L of 1X transfer buffer: mix 50 ml of 20X Transfer Buffer, 100 ml of methanol or ethanol and 850 ml of deionized water.

Compatible Gel Tanks:

GradiGel Mini 4-Cell

IBI Universal Protein System

EC 4-Cell

Hoefer Tall Mighty Small™ (SE 280)

Hoefer Mighty Small™(SE 260/SE 250)

Daiichi Mini 2-Gel & 6-Gel

Owl Road Runner, Penguin

Bio-Rad Mini-PROTEAN™ II & 3

Owl Single Sided Vertical System

Technical Manual: