BacPower^TM Bacterial Expression System

Bacterial Protein Expression System is the most common and economical mean of protein expression available today. However, two major problems often prevent researchers from accessing the system's full advantages. One problem is that the protein of interest cannot always be expressed using the specific vector/strain combination. The other problem is the occasional insolubility of the proteins expressed in bacterial system.

Over the years, GenScript has developed BacPower^TM and FoldArt^TM technologies to tackle hard-to-express and hard-to-dissolve proteins. With BacPower^TM technology, the expression level can be greatly improved for most of the proteins. In addition, our FoldArt^TM protein refolding technology is designed to determine the optimal refolding conditions by evaluation a series of buffers. Over 95% of the inclusion bodies can be refolded and solublized by our proprietary FoldArt^TM protein refolding technology.

Assembled on top of all of these advanced technologies, BacPower^TM Guaranteed Protein Package enables GenScript to deliver 3 mg of purified soluble protein to you in as little as 4 weeks at very affordable prices. If your research project starts from a piece of gene sequence, you can also try our OptimumGene^TM Gene-to-Protein Package which will deliver you 10 mg of guaranteed purified protein for as little as $2,990 and free gene synthesis up to 1 kb.

Advantages:

• Proprietary BacPower^TM and FoldArt^TM technologies
• BacPower^TM Guaranteed Package: guaranteed 3 mg of purified proteins, starting from $2,200
• Fast turnaround: as little as 4 weeks
• Flexible scale-up ranging from 10 L to 1,000 L
• One-stop service platform: GenScript can take your project directly from gene synthesis to downstream activities including recombinant protein expression, stable cell line generation, antibody production, and assay development.
  

• BacPower^TM Guaranteed Package
• BacPower^TM Economic Package
• Case Studies
• Customer Testimonial

BacPowerTM Guaranteed Package (SC1253) Milestones Specifications Deliverables Timeline1 1. Subcloning Subclone the gene into 3 expression vectors (Customer provides the gene or GenScript synthesizes the gene for an additional fee) Construct(s) report 2 weeks 2. Expression optimization and verification Transformation of 3 constructs into bacteria Expression optimization: induction time, temperature, and IPTG concentration Coomassie blue stained SDS-PAGE gel Yield estimation and feasibility proposal for large-scale expression (optional) QC data 1–2 weeks 3. Expression and purification Expression in 1 L bacterial culture One-step affinity purification QC by SDS-PAGE, Western blot2, and MALDI-TOF3 3 mg or more of purified soluble tagged protein QC data 1–2 weeks Total price of BacPowerTM Guaranteed Package: starting from $2,200 1 Listed is the typical turnaround time at GenScript. Please note that additional requirements may incur additional steps, and likewise longer processing time. 2 FREE Western QC data is available for tagged proteins that can be detected by the anti-His or anti-GST antibody. For other tagged proteins, additional charges may apply. 3 Optional MALDI-TOF data is available with additional charge.

BacPowerTM Economic Package (SC1256) Milestones Specifications Deliverables Timeline1 1. Subcloning Subcloning into up to 1 expression construct, under the choice of the client Construct(s) report 2 weeks 2. Pilot expression and purification Follow client's protocol about temperature, time course, inducer concentration Appropriate E.coli strain SDS-PAGE gel and/or Western blot Expression data 1 week 3. Protein expression and one-step purification One strain from above Follow client's purification protocol QC by SDS-PAGE, Western blot2, and MALDI-TOF3 Purified protein QC data 1–2 weeks Total price of BacPowerTM Economic Package: starting from $1,750 1 Listed is the typical turnaround time at GenScript. Please note that additional requirements may incur additional steps, and likewise longer processing time. 2 FREE Western QC data is available for tagged proteins that can be detected by the anti-His or anti-GST antibody. For other tagged proteins, additional charges may apply. 3 Optional MALDI-TOF data is available with additional charge. This service can be expanded to include tag removal, endotoxin removal, and additional custom purification for a separate fee.

1. Protein refolding Overproduction of proteins in E.coli can cause the formation of inactive, misfolded and insoluble protein aggregates. GenScript provides proprietary refolding technology to solve this problem. A case study shows that over 95% of the inclusion body is solublized and refolded. The purity of the refolded protein is more than 85%. The refolded protein is delivered in customized buffer. Fig. A: Refolded protein of sample was analyzed by SDS-PAGE followed by Coomassie Brilliant Blue staining Lane M: Smart Protein Standard (Middle-Range) (GenScript, Cat. No. MM0900) Lane 1: Refolded protein of sample 2. Tag removal Presence of epitope tag in recombinant proteins may result in changes in protein structure, toxicity, and loss of bioactivity. To avoid these problems, tag removal after protein expression is one of additional requests from some customers. Shown below is protein that is cleaved by SUMO protease and successfully isolated. About 80% tag free target protein with purity of >90% is recovered. SDS-PAGE checking purification of protein after tag cleavage by SUMO protease Fig. B: Lane M: Smart Protein Standard (Middle-Range) (GenScript, Cat.No. MM0900) Lane 1: Refolded fusion protein with SUMO-tag Fig. C: Lane M: Smart Protein Standard (Middle-Range) (GenScript, Cat.No. MM0900) Lane 1: Recovered protein after 1st incubation with Ni-resin Lane 2: Recovered protein after 2nd incubation with Ni-resin Lane 3: Elute with 300 mM imidazole from Ni-resin of 2nd incubation

Customer Testimonial "GenScript provides fast, professional protein synthesis services at very reasonable prices. By making it cost-effective to outsource protein production, GenScript has made it possible for my lab to focus on our own area of expertise and get more research done. The detailed planning, updates, and reports that GenScript provides all of the quality control that one could ask for. I strongly recommend GenScript's protein production service." — Dr. Barry Bradford, Kansas State University Department of Animal Sciences & Industry Partial publications cited our protein services Flavia Fontanesi, Iliana C. Soto, Darryl Horn, and Antoni Barrientos. Mss51 and Ssc1 facilitate translational regulation of cytochrome c oxidase biogenesis. Mol. Cell. Biol. Jan 2010; 30(1): 245 - 259 Yanchun Li, Darren F. Boehning, Ting Qian, Vsevolod L. Popov, and Steven A. Weinman. Hepatitis C virus core protein increases mitochondrial ROS production by stimulation of Ca2+ uniporter activity FASEB. J. Aug 2007: 2474 - 2485 More publications

Quotations and Ordering:

To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.

To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure online messaging system.

If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Protein Services, GenScript, 860 Centennial Avenue., Piscataway, NJ 08854, US.

For questions or to inquire about the status of your order, contact us by email, phone, fax or via our secure online messaging system.

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

	Email: protein@genscript.com
	Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
	Fax: 1-732-210-0262 1-732-885-5878